Salts such as NaCl, KCl, and MgCl 2;Contains 5 M guanidineHCl, mM TrisHCl, pH 66 (25°C) (final concentration after addition of ethanol) • 33 ml, add ml absolute ethanol 4 blue Wash Buffer Contains mM NaCl, 2 mM TrisHCl, pH 75 (25°C) (final concentrations after addition of ethanol) • ml add 80 ml absolute ethanol 5 colorless Elution Buffer Water PCR grade • 30 ml All solutions are clear, except Vial 1Only be considered under cases of severe contamination Cleaning is performed at low flow rates using 1 CV of sodium hydroxide (500 mM sodium hydroxide for Superdex and Superose columns and 0 mM sodium hydroxide for Sephacryl columns) Note that the column should never be stored in sodium hydroxide Equilibrate the column immediately after the cleaning with 2 CV of
Monitoring The Purification Ppt Download
